Correlation of metastasis associated gene-1 and mammalian target of rapamycin expression with clinical pathology in rectal cancer / 中国医师进修杂志

Objective To investigate the expression of metastasis associated gene-1 (mag-1) and mammalian target of rapamycin (mTOR) in rectal cancer, and its correlation with clinical pathology. Methods The expressions of mag-1 and mTOR in 60 rectal cancer tissue, 30 adenoma tissues and 10 normal rectal tissues were detected by immunohistochemical method, and the correlation between the expression levels and rectal cancer clinical pathologic characteristics was discussed. Results The positive expression rates of mag-1 and mTOR in rectal cancer tissue were significantly higher than those in normal rectal tissue and adenoma tissue:55%(33/60) vs. 1/10 and 27%(8/30), 58%(35/60) vs. 2/10 and 30% (9/30), and there were statistical differences (P 0.05). The correlation analysis result showed that the expressions of mag-1 and mTOR were positively correlated in rectal cancer tissue (r=0.730, P<0.01). Conclusions The mag-1 and mTOR may correlate with invasion and metastasis in rectal cancer, and monitoring mag-1 and mTOR expression has a certain value for determining biological behavior of rectal cancer.

Correlation of phosphatase and tensin homolog gene and 1-phosphatidylinositol 3-kinase expression with clinical pathology in rectal cancer / 中国医师进修杂志

Objective To investigate the expression of phosphatase and tensin homolog gene (PTEN) and 1-phosphatidylinositol 3-kinase (PI3K) in rectal cancer and its correlation with clinical pathology.Methods MaxVisionTM immunohistochemical method was used to detect the expressions of PTEN and PI3K in 60 rectal cancer tissues,30 adenoma tissues and 10 normal rectal tissues,and the correlation between the expression levels and its clinicopathologic characteristics was discussed.Results The positive expression rate of PTEN in rectal cancer tissue was significantly lower than that in normal rectal tissue and adenoma tissue [43.3％ (26/60) vs.100.0％ (10/10),93.3％ (28/30),P ＜ 0.05].The positive expression rate of PI3K in rectal cancer tissue was significantly higher than that in normal rectal tissue and adenoma tissue [75.0％ (45/60) vs.30.0％ (3/10),33.3％ (10/30),P ＜ 0.05].The positive expression of PTEN and PI3K were correlated with carcinoembryonic antigen,degree of cell differentiation,TNM stage,lymph node metastasis and distant metastasis(P＜ 0.01 or ＜ 0.05 ),but had no correlation with age,gender,neoplasm location,neoplasm appearance (P ＞ 0.05).The correlated analysis showed that the positive expression of PTEN had negative correlation with PI3K (r =-0.269,P =0.023 ).Conclusions The high expression of PI3K and down regulation of PTEN in rectal cancer are closely related to its invasion and metastasis.There are some values to monitor PTEN and PI3K expression for determining biological behavior of rectal cancer.